Evaluation of apoptotic effect of crocin, cisplatin, and their combination in human oral squamous cell carcinoma cell line HN5

Background: Squamous cell carcinoma (SCC) is the most common oral malignancy with high rate of mortality. Cisplatin, as effective chemotherapy drug, has side effects. Considering studies on use crocin in saffron treatment various malignancies, this study aimed at investigating effects and cisplatin their combination SCC fibroblast lines. Materials Methods: In interventional study, HN5 lines were treated different concentrations (12.5–50 μg/mL) (2, 4, 8, 16, 32 μg/mL), cells counted after 24, 48, 72 h by 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide assay. Data analyzed SPSS Version 17, P < 0.05 was considered level significance. final stage, flow cytometry 24 terms pattern death done. Results: Both drugs had a toxic effect malignant cells. One point 8 μg/mL not only cancer (P 0.001) but also fibroblasts. However, 12.5 same line, despite less fibroblasts comparison alone = 0.012). Apoptosis showed cytometry. Conclusion: Crocin can have significant toxicity healthy tissue. It seems that lower doses crocin, cisplatin, besides having anticancer effect, reduce Key Words: Apoptosis, carcinoma, culture techniques, crocus, squamous